Single cell RNA sequencing (scRNA-seq) has revolutionized the way we study gene expression at the cellular level. By sequencing the RNA of individual cells, we can now gain a deeper understanding of ty and biological processes at the single cell level.
However, with the abundance of data generated by scRNA-seq experiments, come the challenges that must be overcome to unlock its full potential.
Discovery teams working on single-cell data typically get stuck for days and weeks on the initial step of sourcing relevant datasets from open-source portals. Storing and analyzing this data is another roadblock.
Let’s take a quick glance at the recurring challenges scientists face while performing single-cell analysis (SCA) and some solutions that could streamline their discovery process.
Standard metadata fields such as tissue, disease, number of samples, platform or sequencing technology (10x or smartseq), organism, sample cohorts, cell types are some of the key annotations that would ease the effort of identifying relevant datasets.
A cloud platform that can store different formats of data such as h5ad or h5seurat, perform compute-intensive processing workflows such as Cellranger, Scanpy, Seurat as well as integrations with open source algorithms such as Nichenet (Ligand-Receptor analysis), CCA, Harmony (Batch Correction), SingleR, SCSA (Automated Cell Type Annotation) or applications would be ideal for processing the data.
A standard single-cell analysis workflow (such as Scanpy, Seurat) should be used to perform analysis across all the datasets so that comparative studies can be carried out between single cell data from different sources. The datasets can then be stored in a single format, such as h5ad format, which is a widely used format in the single-cell sequencing community. It should be designed to store large amounts of data and allow fast querying of parts of a file without accessing the complete file in memory.
A resource/ repository that collates all the single cell data on diverse areas, especially oncology, will save a lot of time and effort for the researchers who could use it to derive meaningful insights.
Interactive visualization tools can help researchers to quickly identify and remove outliers or low-quality cells, reducing the time and effort required for manual curation. These tools allow researchers to visualize their scRNA-seq data in a variety of ways, making it easier to identify and remove outliers or low-quality cells.
Collaboration and sharing of scRNA-seq data and curation tools among researchers can play a significant role in streamlining the curation process for single-cell RNA-seq data analysis.
In conclusion, curation is a critical step in scRNA-seq data analysis and should not be overlooked. By organizing, cleaning, and standardizing the data, curation helps to ensure that the results of scRNA-seq data analysis are accurate and reliable. As the field of scRNA-seq continues to grow and evolve, the importance of curation will only increase, making it a key component of the data analysis pipeline.
Elucidata’s data-centric ML Ops platform, Polly, allows the user to carry out integrative analysis on single-cell data. We have the world’s largest collection of ML-ready single-cell and bulk RNA seq data. Polly hosts highly curated datasets following standard ontologies with harmonized metadata, standardized and normalized data processed through consistent pipelines, and accurate expert-annotated cell types to ensure reliable results and to empower scientists in achieving their research goals.
Reach out to us or email us at info@elucidata.io to learn more!