Single-cell RNA-seq

Harmonize in-house and public single-cell RNA-seq datasets to ML-ready formats, and leverage our suite of custom solutions designed for scRNA-seq data to accelerate analysis and insight generation.

Technology

Transform Disparate Single-cell RNA-seq Data
into Actionable Insights

Why Choose Elucidata for Your Single-cell Research?

Custom-curated datasets for your unique research objectives.

Diverse single-cell modalities to expand your research horizons.

Expert QC & analytics to support your data.

Elevate your data with expert metadata curation.

Case studies

Pharma Company Achieves 4x Faster Target ID for Inflammatory Disease

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Case studies

Accelerating Immune Disorder Research with 5M Harmonized Cells

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Whitepaper

Leveraging Machine Learning for Robust Cell Type Annotation: A Data-Driven Perspective

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Accelerate Time to Insights with Curated Single-cell Data

Configure Curation to Fit Your Analysis Needs

Harmonize unstructured single-cell data with a customizable, transparent curation process tailored to your criteria for faster analysis, supporting modalities like CITE-Seq, Spatial, ATAC-Seq, and more.

Curated collection of High-Quality Datasets

Our harmonized and curated single-cell datasets undergo rigorous standardization and quality control, ensuring uniformity and reliability across all samples.

Advanced Single-Cell Modalities

Leverage our harmonized and curated datasets across single-cell modalities such as CITE-Seq, Spatial Transcriptomics, and ATAC-seq, to gain deeper insights into cellular functions, tissue architecture, and gene regulation at an unprecedented resolution.

Unify and Manage Data from In-house Assays on a Single Atlas

Integrate multi-modal datasets into a single, unified Atlas to uncover hidden patterns and accelerate research breakthroughs.

Custom Data Processing Pipelines

Access raw counts from original publications, obtain consistently processed single-cell data, or replicate author-defined counts to suit your research needs.

Cell Type Annotation

Leverage our harmonization engine for custom cell type annotations using markers derived from sub-clusters or figures in relevant publications.

Comprehensive and Rigorous Quality Checks

Our single-cell datasets undergo a robust 50-step QA/QC process, ensuring high metadata quality, filtering, normalization, batch effect correction, and accurate measurement quality.

Derive Faster Insights from Harmonized Single-cell Data

Utilize our comprehensive suite of machine learning (ML) solutions to extract insights faster and streamline your downstream analysis.

Harness the Synergy of ML for 75% Faster Insights

Collaborate with our experts to deploy popular foundational models, such as scGPT, on your harmonized data, or fine-tune existing models to enhance predictions and accelerate insights.

Network Analysis and Use-cases for Bioinformatics

Utilize tools for constructing and analyzing cell-cell interaction networks, providing insights into cellular communication and signaling pathways. Perform advanced analyses, including differential expression, trajectory analysis, UMAP, clustering, and more.

Use GPT for Querying and Analysis

Run complex queries across your harmonized data using GPT, a natural language querying interface that performs sophisticated statistical analyses, such as PCA and differential gene expression.

Analyze, Visualize, and Explore Single-cell Data

Leverage our expertise to create custom data consumption workflows unique to your research.

Visualize Harmonized Data Using CellxGene

Utilize integrated web applications, such as CellxGene and CellxGene VIP, to analyze and visualize single-cell data in real time.

Build, Deploy, and Maintain Custom Apps
or Dashboards

Stream harmonized data to external tools like Spotfire or integrate it with your preferred analysis environments, such as React or Shiny.

Multi-omics Integration

Develop methods to integrate single-cell data with other omics data types (e.g., genomics, proteomics) for a more comprehensive understanding of cellular processes.

Unraveling Cellular Heterogeneity with
Single-Cell Data Analysis

Case study: Accelerated Target ID using ML-Ready data on Polly

Immune Cell Characterization

Leverage scRNA-seq to dissect diverse immune cell populations within tumor microenvironments. This analysis reveals mechanisms of immune evasion and identifies novel therapeutic targets, enhancing the potential for effective cancer immunotherapies.

Case study: Accelerated Target ID using ML-Ready data on Polly

Cell Lineage and Differentiation Mapping

Utilize single-cell transcriptomics to trace the developmental pathways of specific cell types in both healthy and diseased tissues. By mapping these trajectories, we gain insights into lineage differentiation and disease progression, aiding in the development of targeted therapies.

Case study: Accelerated Target ID using ML-Ready data on Polly

Personalized Drug Response Evaluation

Analyze individual cell responses to therapeutic agents within heterogeneous populations to identify resistant subpopulations. This critical evaluation informs personalized treatment strategies, ensuring therapies are tailored to meet the unique challenges posed by each patient’s disease.

Trusted by World's Leading Biopharma Companies

Testimonials

Harmonized Single-Cell Datasets to Enhance
Precision Medicine Initiatives

Harness single-cell insights to unravel intricate cellular heterogeneity, immune responses, and developmental pathways, driving innovative therapeutic strategies in precision medicine through advanced scRNA-seq analysis.

Data processing and scientific reporting of metabolomics data are crucial but also tedious. Polly’s range of applications helped our team in accelerating these processes significantly and effectively.

Dewakar Sangaraju
Senior Scientist,
Genentech

Extremely impressed by Polly and its power to digest and integrate large datasets. The unique partnership that we have allows us to work hand-in-hand with software engineers and scientists to rapidly develop novel hypotheses that we can test in the lab.

Dr. Kate Yen
Founder & CEO,
Auron Therapeutics

We really were pleasantly surprised because this tool [El-MAVEN] is really useful in fast analysis of tracer data. It allows us to really look at a broad set of targets so you are not limited anymore for data analysis. A data analyst of mine stepped up to me and said, "I can do now in 3 hours what used to take 4 days.

Bart Ghesquière
Head of Metabolomics
Core Facility, VIB

Data analysis is the biggest bottleneck for metabolomics research. Elucidata has worked directly with us to streamline our analysis pipeline, accelerating the pace of our research.

Dr. Russel Jones
Lead Investigator,
Van Andel Institute

We were looking for public data on a tight deadline to make decisions on our back up targets for solid tumors. Elucidata was able to understand our relevance criteria, identify, and deliver high-quality harmonized data ahead of schedule. We are really happy with their deliveries and this partnership.

Ming 'Tommy' Tang
Director of Computational Biology,
Immunitas Therapeutics
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FAQs

What are the quality measures applied to single-cell datasets on Polly?

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  1. We perform 50+ quality checks on single-cell datasets on Polly to ensure high-quality datasets are delivered. Broadly, the checks are categorized as follows:
  • Metadata checks ensure metadata follows the specified ontology, no missing metadata, no missing samples, source link addition, etc.
  • Data matrix checks to ensure that data is properly processed, clustered, and annotated.
  1. We share a quality report for each dataset that is processed on Polly which contains the processing and quality details.

What metadata fields are curated for single-cell RNA-seq data on Polly? Can users request the curation of additional metadata fields?

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We offer 30+  curated fields for single-cell RNA-seq datasets on Polly. If any additional curated fields are required, they are added on request as part of custom curation.

In what formats are single-cell datasets on Polly provided, and are they compatible with common bioinformatics tools and pipelines?

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Single-cell datasets are stored in the H5AD format on Polly. Additionally, our team can also support custom requests for providing data in the file formats that are best suited for the downstream bioinformatics tools and pipelines used by our clients.

Can single-cell data on Polly be accessed and downloaded? Do you support cloud-based access or direct transfer?

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  1. Single-cell data on Polly can be easily accessed and downloaded via the GUI or the Polly Python module. The downloaded file will be in the H5AD file format and will contain both, the data matrix and the metadata.
  2. On request, our team can build exporters that will transfer data on Polly to customers' cloud storage as a service.

What are the benefits of using single-cell on Polly for single-cell analysis?

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Single-cell data on Polly has various benefits,

  1. Access high-quality data.
  2. Transparency in dataset processing.
  3. Customizable metadata harmonization.
  4. Transparency in dataset processing.

What distinguishes Polly's processed single-cell datasets from unfiltered raw counts?

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  1. Polly processed single-cell data is consistently processed using a validated scanpy-based pipeline which takes author-provided raw counts data as input and gives filtered, normalized, clustered and cell-type annotated data as output. All Polly-processed single-cell datasets have cell type annotation available as per author-provided markers and have two H5AD files available:
  • Polly Processed H5AD - Containing normalized counts and metadata including cell type annotation.
  • Raw Counts H5AD - Containing raw/integer counts and sample metadata.
  1. Unfiltered raw counts on Polly are integer counts that are not filtered and normalized. Clustering cell type annotation is available if provided by the author at the source. Clustering and cell type annotation is not exclusively performed and might not be available for all raw count datasets. There will be only one H5AD file available.
  • Raw Counts H5AD - Containing raw/integer counts and sample metadata.

How do you do cell type annotation for single-cell datasets on Polly? Is it customizable?

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  1. We perform scType-assisted cell type annotation on Polly using the cell type markers as documented in the publication of the dataset.
  2. If required, our team can add the following customizations for cell-type annotation
  • Using cell type markers list as specified by our clients.
  • Customization of the cell type annotation methodology as service.

Do you provide integrated single-cell data on Polly? Is there a particular integration methodology you follow?

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Yes, our team has the expertise to provide integrated single-cell datasets on Polly upon request. We don't follow a specific method for integration. The integration methodology is architected by our team of experts based on the biological question and the downstream analysis our client wants to perform.

What are the single-cell data processing pipelines used on Polly? Do you offer customization?

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  1. We primarily use a scanpy-based validated pipeline to process single-cell datasets on Polly. This uses the author-provided raw counts as the starting point, performs filtering low-quality genes/cells, doublet filtering, normalization, batch effect removal from samples within a dataset (not across datasets), clustering, and cell type annotation.
  2. There is also a Cell Ranger pipeline we have in place for processing fastq files generated using the 10x platform. This can be used on request and will be restricted to datasets have fastq files available at source and are generated using the 10x platform.
  3. We offer various customizations such as
  • Customizations to the parameters of the scanpy-based single-cell pipeline.
  • Customizations to the tools and algorithms being used in the two pipelines mentioned above as service.
  • Building a pipeline from scratch as per client requirements as service.
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