CRISPR Cas9 is a powerful gene-editing tool that cuts the DNA in a precise manner. The most common use of CRISPR Screening is finding gene targets for various diseases. The process of a typical CRISPR Screening experiment is as follows :
Typical CRISPR Screening experiment
There are various tools developed for processing CRISPR Screening data. Some of them are MAGeCK, HitSelect, ScreenBEAM, BAGEL, sgRSEA, PBNPA, MAGeCK VISPR which use different algorithms. All these tools are script based which can be used only by bioinformaticians or tech-savvy biologists. These are not friendly to most scientists. Apart from this, there were some other challenges as well that were faced by scientists and even bioinformaticians. Let’s try to understand the challenges taking the example of one of the most widely used tools for CRISPR Screening- MAGeCK.
MAGeCK VISPR was developed in order to overcome some of the shortcomings of MAGeCK. It uses the base algorithm of MAGeCK and does everything that MAGeCK does. On top of that, it has some additional algorithms and features that make it much more useful than MAGeCK. Those features are:
Even though MAGeCK VISPR was a significant improvement over MAGeCK, there were some critical limitations that persisted.
Polly CRISPR Screening, based on MAGeCK algorithm, is a cloud-based tool with an interactive interface instead of a command-line interface. This decreases the learning curve of the tool and makes it much easier to use. And, considering it is cloud-based, there’s no need to install anything on the local computer and data can be processed from any computer.
Polly CRISPR Screening Workflow
The benefits of using Polly CRISPR screening workflow are:
We hope to add more features to the tool to make it even more useful. One such key feature we believe would add a lot of value is giving related publications to the ongoing study. Suppose you find that gene “XYZ” is of some importance to disease “ABC”. You can get a list of publications which relate the mentioned disease to the mentioned gene. This makes it much faster to accept or reject the hypothesis.You read how Polly CRISPR screening workflow has a huge edge over anything else available for CRISPR screening studies. Now it is time you check it out yourself.
To experience Polly CRISPR Screening Workflow, book a quick demo here.
Get the latest insights on Biomolecular data and ML